For Sale Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector
Pre-owned instrument
• Fully Functional
• Current model
• Serviced & in Stock
• Good cosmetic condition
• Warranty
• An extended warranty option and training may be available.
Here is what you will receive:
• Acquity H bioQuaternary Solvent Manager (bio-QSM #186015041)
• Acquity H bioSample manager-FTN with 50uL extension loop (bio SM-FTN #186015040)
• Acquity TUV detector (TUV #186015031) w/ Analytical 500nL Flow Cell
• Acquity Column heater/cooler 30cm (30-cm CHC #186015011)
• Acquity Column heater (CH-A #186015042)
• Solvents Tray
• Inter-communication cables
• Standard power cords.
Please Note: Computer and Empower software licenses will need to be purchased separately. They are not included. The computer was used for testing only.
Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector Configuration:
• Dwell volume (total system): <400 uL (includes standard 100 uL mixer)
• Integrated leak management: leak sensors, as standard, and safe leak handling
• Quantum synchronization: injection synchronization between the pump and sample manager enhances retention time reproducibility
• Settable flow rate range: 0.001 to 2.200 mL/min in 0.001 mL increments
• Maximum operating pressure: 15,000 psi up to 1.0 mL/min, 7800 psi up to 2.2 mL/min
• pH range: 2 to 12
• Unattended operation: leak sensors and safe leak handling, full 96-hour diagnostic data display through console software
• Cycle time: <30 s inject-to-inject
BioQuaternary Solvent Manager:
• Number of solvents: up to four solvents in any combination
• Solvent degassing: Integrated vacuum degassing, four chambers; one additional chamber for the SM-FTN purge solvent
• Solvent blending: automated, on-line pH, ionic strength, and organic modifier blending from pure solvents with Auto-Blend Plus Technology
• Gradient formation: Low-pressure mixing, quaternary gradient
• Gradient profiles: 11 gradient curves [including linear, step (2), concave (4), and convex (4)]
• Primary check valve: Intelligent Intake Valve (i2 Valve)
• Flow accuracy ±1.0% at 0.5 to 2.0 mL/min using 100% A (with i2 Valve); Backpressure 600 to 1000 psi with degassed H20
• Flow precision 0.075% RSD or ±0.020 min SD, whichever is greater, based on six replicates (with i2 Valve); H2O/MeOH (60:40) via Auto-Blend Plus Technology
• Compressibility compensation: automatic and continuous
• Priming: wet priming can run at flow rates up to 4 mL/min
• Pump seal wash: equipped with an automated wash system to flush the rear of the high pressure seal and the plunger
• Flow ramping Range: 0.01 to 30.00 min to reach 2.00 mL/min; Default: 0.45 min to reach 2.00 mL/min
BioSample manager-FTN:
• Injection volume range: 0.1 to 50 uL, up to 1,000.0 uL with optional extension loops
• Number of sample plates: any two of the following: 96 and 384 microtiter plates, 48 position 2.00-mL vial plates, 48 position 0.65-mL micro-centrifuge tube plates, 24 position 1.50-mL micro-centrifuge tube plates
• Maximum sample capacity: 768 in two 384-well plates or 96 in 2-mL vial holders, four additional positions for dilution functions
• Sample compartment temperature range: 4DegC to 40DegC, settable in 0.1 DegC increments
• Temperature accuracy: ±0.5DegC at sensor
• Temperature stability: ±1.DegC at sensor
• Injection needle wash: Integral, active, programmable
• Minimum sample required: 3 uL residual, using total recovery 2-mL vials (zero offsets)
• Advanced Sample Manager capabilities: auto-dilution, auto-addition, and load-ahead
UPLC TUV Detector:
• Wavelength range: 190 to 700 nm
• Wavelength accuracy: ±1 nm
• Sampling rate: up to 80 points/s
• Light source: deuterium lamp
• Flow Cell: Analytical 500nL Flow Cell
• Dispersion volume: ≤0.8 uL (analytical flow cell);
• Flow cell design: light-guiding UPLC intelligent technology flow cell
• Path length: 10 mm (analytical flow cell)
• Flow cell volume: 500 nL (analytical flow cell)
• Pressure limit: 1000 psi (all flow cells)
• Optical bandwidth: 5 nm
Column heater/cooler 30cm:
• Column capacity: accommodates multiple columns, 20 to 300 mm in length, between 2.1 - 7.8 mm internal diameter (I.D.)
• Compartment temperature control: 4-65DegC in 0.1DegC increments
• Column compartment temperature accuracy: ±0.8 DegC at the sensor
• Compartment operational temperature range: cooling: 15 DegC below ambient; heating: 5DegC above ambient to 65DegC
Column heater:
• Column capacity: Single column, up to 150 mm in length, up to 4.6 mm internal diameter (I.D.)
• Column compartment temperature range: 5-90DegC, settable in 0.1DegC increments
• Column compartment temperature accuracy: ±0.5 DegC at the sensor
• Column compartment temperature stability ±0.3 DegC at the sensor
• Solvent conditioning: active pre-heating
Customization/Options:
Other detectors, flow cell options, and sample loops might be available. Please inquire with our sales team.
Testing performed by our in-house service team:
• We inspect the Waters Acquity UPLC-H Bio Liquid Chromatography System for physical damage.
• We check for seamless communication with the Empower software.
• We run samples and tests that allow us to perform IQ/OQ/PQ.
BioQuaternary Solvent Manager:
• We check to ensure the proper initialization of the Solvent Manager.
• We thoroughly assess the stability and consistency of solvent delivery.
• We verify the accuracy of flow rates.
• We evaluate the system's performance in running gradient tests.
• We test the functionality and performance of the pressure limiter.
• We verified that the seal-washing pump is operational efficiently.
• We perform degasser health checks to verify the degasser is functioning correctly.
bioSample manager-FTN:
• We check to ensure the proper initialization of the sample manager.
• We evaluate the accuracy of the sample cooler set temperature for consistency and precision.
• We verify the reproducibility of sample handling processes.
• We check the column oven's temperature accuracy within specified standards to ensure precise control for optimal chromatographic separation.
• We check the syringe's integrity and perform a Sample Syringe Leak Test.
• We inspect the integrity of the needle seal by performing the Needle Seal Readiness Test.
• We perform a quick test of the leak detector.
• We perform the system's reproducibility tests, ensuring consistent and reliable performance across multiple runs and providing confidence in the accuracy of chromatographic results.
TUV detector:
• We perform checks to evaluate the proper initialization of the TUV detector.
• We check the remaining lamp life to ensure optimal performance.
• We run QC sample tests to verify accurate and reliable detection capabilities.
Column heaters:
• We run the initialization of the column heater.
• We run tests to verify the accuracy and stability of the column oven temperature.
• We perform ramping stress tests to verify performance.
Instrument Overview:
• Waters Acquity UPLC H-Class Bio Liquid Chromatography System provides high-performance separation and analysis capabilities.
• Equipped with a TUV Detector to enable sensitive detection in various applications.
• Offers advanced features for precise quantification and identification of compounds.
• Streamlined design for efficient sample processing and data acquisition.
• Reliable performance is ideal for bioanalytical and pharmaceutical research labs.
• Ensures optimal chromatographic results with enhanced sensitivity and speed.
Instrument Application:
• Peptide mapping
• Ion exchange
• Size exclusion
• Hydrophilic interaction
• Reversed-phase
• Glycan analysis
• Oligonucleotide analysis
The key features of Waters Acquity UPLC H-Class Bio Liquid Chromatography System with TUV Detector:
• Advanced separation
• Sensitive TUV detection
• Auto-Blend Plus technology
• Multi-solvent blending
• Accurate column temperature control
• Modular design
• Reliable bioanalysis
Additional technical info:
TUV Detector Flow cell options:
• Analytical: 500 nL volume, 10 mm pathlength
• High sensitivity: 2400 nL volume, 25 mm pathlength
• Stainless steel: 1.5 uL volume, 5 mm pathlength
• Titanium: 1.5 uL volume, 5 mm pathlength
• Low dispersion analytical flow cell: 500 nL, 10 mm pathlength
• Low dispersion microbore flow cell: 250 nL, 10 mm pathlength
• Microscale: 100 nL, 10 mm pathlength
Why should you buy from us?
Our Advantage
• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping.
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• Remote installation and instrument operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)
Our Guarantee
• It will work!
• Warranty
• Smooth process from the initial quote to order fulfillment.
• Customer references are available.
This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
*SM* Small Crate
300#
PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE
Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.
Q: What about the warranty?
A: The instrument comes fully warranted for both parts and labor.
Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to familiarize you with this instrument.
Q: What should I watch out for when buying a used/pre-owned instrument from another lab?
A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.
Q: Do you have more than one of these instruments on hand?
A: We generally carry multiple instruments on hand, along with a variety of spare parts.
Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?
A: We accept purchase orders from all major US and Canadian universities.
Q: How fast will you ship?
A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.
Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?
A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.
Q: Do you ship Internationally?
A: Yes! We have shipped to multiple continents and over 30 countries.
Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new instrument, you will save 60% or more and have plenty of funds left over for an additional instrument or two.
TO VIEW ADDITIONAL PHOTOS, VIDEOS AND VERY DETAILED INFORMATION SUCH AS TEST STEPS PERFORMED, CONFIGURATION, ETC., PLEASE VISIT OUR WEB SITE. TO GET THERE PLEASE CLICK ON “WEBSTORE”; TO YOUR RIGHT AND YOU WILL SEE LINK TO OUR SITE IN THE MIDDLE OF THE SCREEN. THEN USE LAST 5 DIGITS OF MPN NUMBER IN THE SEARCH FIELD TO LOCATE YOUR ITEM.
DO NOT HESITATE TO CALL US OR EMAIL US WITH ANY QUESTIONS YOU MIGHT HAVE.
ALL OF OUR ITEMS ARE AVAILABLE FOR INSPECTION AT OUR FACILITY.
Return Policy
Items are sold as-is with no returns or refunds available unless explicitly stated.
