Here is a BD LSRFortessa X-20 Cell Analyzer Flow Cytometer (4)Lasers(16)Colors/(18)Detectors with HTS
Predecessor to:
Current model
Condition:
This BD LSRFortessa Cell Analyzer Flow Cytometer is in good working condition.
Please see the exact video and photos below.
Testing we performed:
The BD LSRFortessa Cell Analyzer Flow Cytometer passed all tests and was found to be fully functional:
Connected to software (Diva 9): PASSED
Ran fluidics priming and sequences: PASSED
Ran several cleaning cycles (long clean, flow cell clean, etc.): PASSED
Tested HTS functionality: PASSED
Performed Optical Alignment: PASSED
(Homing, Priming, Cleaning): PASSED
Tested run with 8 Peak Beads: PASSED
System consists of:
BD LSRFortessa Flow Cytometer
HTS High Throughput Sampler
Computer with FACSDiva software ver9.0 w/ Dongle (with monitor, keyboard, mouse)
Communication and power cables.
Configuration and Setup:
Optics:
(4) Lasers: Violet (405nm), Blue (488nm), Yellow/ Green (561nm), Red (650nm)
(16) Colors simultaneously
(18) Detector Channels
- Forward Scatter Detection: Photodiode detector with a 488/10 BP filter
- Side Scatter Detector: Photomultiplier tube (PMT) with a 488/10 BP filter
- 405 violet laser detection channels: A (780/60, 750LP), B (710/50, 690LP), C (670/30, 635LP), D (610/20, 600LP), E (525/50, 505LP) F (450/50, GG420)
- 488 blue laser detection channels: A (710/50, 685LP), B (530/30, 505LP)
- 561 yelllow/green laser detection channels: A (780/40, 750LP), B (710/50, 685LP), C (670/30, 635LP), D (610/20, 600LP), E (586/15)
- red laser detection channels: A (780/60, 750LP) B (730/45, 690LP), C (670/30, RG665)
Fluorescence Sensitivity:
- FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC)
- PE: 30 molecules of equivalent soluble fluorochrome (MESF-PE)
- PE-Cy5: 10 molecules of equivalent soluble fluorochrome (MESF-PE Cy5)
- APC: 70 molecules of equivalent soluble fluorochrome (MESF-APC)
Fluorescence Resolution: Coefficient of variation PI: Area of <3%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
Fluorescence Linearity: Doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488-nm blue laser
Data Acquisition Rate: 40,000 events/sec with beads
Fluidics:
Sample Flow Rates: Front button panel provides three modes: RUN, STANDBY, and PRIME
Continuously adjustable flow rate, plus three preset flow rates: LO: 12 uL/min; MED: 35 uL/min; HI: 60 uL/min
Dimensions (H x W x D): 76.2 x 73.7 x 76.2 cm (30 x 29 x 30 in.)
Weight: ~159 kg (~350 lb)
High Throughput Sampler:
The BD High Throughput Sampler (HTS) is available to increase your lab productivity by acquiring samples from a 96- or 384-well microtiter plate.
HTS Throughput Acquisition: less than 15 minutes per microtiter plate in high-throughput mode using a 2-second acquisition, less than 44 minutes in standard mode using a 10-second acquisition
Carryover: <0.5% High-throughput mode; <0.75% Standard mode
Video Description:
The video below gives an overview of the BD LSRFortessa Cell Analyzer Flow Cytometer, and also demonstrates its functionality.
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