The probe is made up of two components that can host fluorescent (glowing) molecules - one 'donor' that transfers the fluorescent molecules to the 'acceptor' component. When the probe comes into contact with HO-1, the bond between the two components is severed, leading to the build-up of the fluorescent molecules in the donor component.
This build-up causes an increase in the fluorescence intensity of the probe that can be detected using spectroscopy. In tests using modified E. coli cells containing human HO-1, the team detected a six-fold increase in the fluorescence of the probe.
Professor James Leiper, associate medical director at the BHF, said: "Current methods to detect IPH rely on hospital-based imaging techniques that are both time consuming and expensive. The current technology aims to produce a fast and sensitive diagnostic test that can be used at the time that a patient first presents with symptoms to allow early detection of IPH. Use of such a test would allow for more rapid treatment and improved outcomes for patients suffering from IPH."
The team are now extending their studies to mammal and human cells. They have recently patented their probe and have received funding from the British Heart Foundation to make a new generation of probes for other cardiovascular and neurodegenerative diseases, and to carry out more in-depth biological investigations of the underlying mechanisms.
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